Challenges in the determination of two analytes with different physicochemical properties, Tapinarof and its main metabolite, Tapinarof Sulfate, at the low picogram level

The challenge of developing a new bioanalytical method considerably increases when two or more metabolites need to be simultaneously analysed. Their extraction and chromatography optimization can be further complicated if their physicochemical properties vary significantly. Method development may be even more difficult when low limits of quantitation are required, for instance, when evaluating the systemic adsorption of topical drugs.

In the work we present to the 8th EBF Young Scientist Symposium, which was awarded with the "Second Best Poster Award", we show the challenges encountered and the potential solutions of developing a fairly sensitive method, while trying to simultaneously extract and analyse two analytes, Tapinarof and, its main phase-II metabolite, Tapinarof Sulfate, whose physicochemical properties are quite different.

You may find below the poster for your ready reference:


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